Journal: European Journal of Medicinal Chemistry

Article Title: Discovery of novel small molecule inhibitors of S100P with in vitro anti-metastatic effects on pancreatic cancer cells

doi: 10.1016/j.ejmech.2020.112621

Figure Lengend Snippet: Active hits, with estimated IC 50 values, from virtual screening of the pharmacophore derived from the putative binding site of cromolyn, 1 , against MOE databases.

Article Snippet: 14 , InterBioScreen STOCK5S-33478 , Image 14 , 77.0.

Techniques: Derivative Assay, Binding Assay

Journal: Journal of oral biosciences

Article Title: Scardovia wiggsiae and its potential role as a caries pathogen

doi: 10.1016/j.job.2017.05.002

Figure Lengend Snippet: (a) Detection of S. mutans or S. wiggsiae in children with S-ECC (red) or caries-free (blue) by anaerobic culture of oral samples on acid medium [26]. (b) Percent of children with detectable S. mutans, S. wiggsiae or a combination of both by species-specific PCR [34]. * = p <0.05; ** = p≤0.01.

Article Snippet: S. wiggsiae could thus not mitigate the effects of local acid production in plaque biofilms. table ft1 table-wrap mode="anchored" t5 caption a7 Peptone Yeast Broth Initial pH of 7.0 Glucose Sucrose Fructose Bacteria Strain pH 7.0 pH 7.0 pH 7.0 Streptococcus mutans ATCC 25175 T 3.83 3.76 3.79 Streptococcus mutans SJ 3.86 3.82 3.8 Streptococcus sobrinus ATCC 27352 T 3.72 3.73 3.74 Streptococcus sobrinus ATCC 33478 2.98 2.97 2.91 Scardovia wiggsiae DSM 22547 / C155A T 3.51 3.26 3.23 Scardovia wiggsiae F0424S 3.3 3.36 3.12 Actinomyces israelii A87A37 4.65 4.66 4.66 Actinomyces naeslundii I H101A18 3.8 3.82 3.87 Actinomyces naeslundii II H403B5 4.69 4.56 4.68 Peptone Yeast Broth Initial pH of 5.5 Glucose Sucrose Fructose Bacteria Strain pH 5.5 pH 5.5 pH 5.5 Streptococcus mutans ATCC 25175 T 3.95 3.87 3.89 Streptococcus mutans SJ 4.11 4.05 4.06 Streptococcus sobrinus ATCC 27352 T 3.99 4.01 4.03 Streptococcus sobrinus ATCC 33478 3.78 3.71 3.68 Scardovia wiggsiae DSM 22547 / C155A T 3.47 3.65 3.52 Scardovia wiggsiae F0424 S 4.02 3.85 3.75 Actinomyces israelii A87A37 3.85 4.09 4.02 Actinomyces naeslundii I H101A18 4.21 4.21 4.12 Actinomyces naeslundii II H403B5 4.57 4.56 4.59 Open in a separate window T=Type strain S=sequenced strain All other strains are clinical isolates Broth media was prepared using Peptone Yeast base and pH adjusted to pH 7 or pH 5.5 and either unsupplemented or with final concentrations of glucose (1%), sucrose (1%) or sucrose (1%).

Techniques:

Journal: Journal of oral biosciences

Article Title: Scardovia wiggsiae and its potential role as a caries pathogen

doi: 10.1016/j.job.2017.05.002

Figure Lengend Snippet: Acid tolerance and Arginine Deiminase Activity of Scardovia wiggsiae , S. mutans, S. sanguinis and A. israelii.

Article Snippet: S. wiggsiae could thus not mitigate the effects of local acid production in plaque biofilms. table ft1 table-wrap mode="anchored" t5 caption a7 Peptone Yeast Broth Initial pH of 7.0 Glucose Sucrose Fructose Bacteria Strain pH 7.0 pH 7.0 pH 7.0 Streptococcus mutans ATCC 25175 T 3.83 3.76 3.79 Streptococcus mutans SJ 3.86 3.82 3.8 Streptococcus sobrinus ATCC 27352 T 3.72 3.73 3.74 Streptococcus sobrinus ATCC 33478 2.98 2.97 2.91 Scardovia wiggsiae DSM 22547 / C155A T 3.51 3.26 3.23 Scardovia wiggsiae F0424S 3.3 3.36 3.12 Actinomyces israelii A87A37 4.65 4.66 4.66 Actinomyces naeslundii I H101A18 3.8 3.82 3.87 Actinomyces naeslundii II H403B5 4.69 4.56 4.68 Peptone Yeast Broth Initial pH of 5.5 Glucose Sucrose Fructose Bacteria Strain pH 5.5 pH 5.5 pH 5.5 Streptococcus mutans ATCC 25175 T 3.95 3.87 3.89 Streptococcus mutans SJ 4.11 4.05 4.06 Streptococcus sobrinus ATCC 27352 T 3.99 4.01 4.03 Streptococcus sobrinus ATCC 33478 3.78 3.71 3.68 Scardovia wiggsiae DSM 22547 / C155A T 3.47 3.65 3.52 Scardovia wiggsiae F0424 S 4.02 3.85 3.75 Actinomyces israelii A87A37 3.85 4.09 4.02 Actinomyces naeslundii I H101A18 4.21 4.21 4.12 Actinomyces naeslundii II H403B5 4.57 4.56 4.59 Open in a separate window T=Type strain S=sequenced strain All other strains are clinical isolates Broth media was prepared using Peptone Yeast base and pH adjusted to pH 7 or pH 5.5 and either unsupplemented or with final concentrations of glucose (1%), sucrose (1%) or sucrose (1%).

Techniques: Activity Assay

Journal: Journal of oral biosciences

Article Title: Scardovia wiggsiae and its potential role as a caries pathogen

doi: 10.1016/j.job.2017.05.002

Figure Lengend Snippet: Acid production of Scardovia wiggsiae, S. mutans, S. sobrinus and Actinomyces species.

Article Snippet: S. wiggsiae could thus not mitigate the effects of local acid production in plaque biofilms. table ft1 table-wrap mode="anchored" t5 caption a7 Peptone Yeast Broth Initial pH of 7.0 Glucose Sucrose Fructose Bacteria Strain pH 7.0 pH 7.0 pH 7.0 Streptococcus mutans ATCC 25175 T 3.83 3.76 3.79 Streptococcus mutans SJ 3.86 3.82 3.8 Streptococcus sobrinus ATCC 27352 T 3.72 3.73 3.74 Streptococcus sobrinus ATCC 33478 2.98 2.97 2.91 Scardovia wiggsiae DSM 22547 / C155A T 3.51 3.26 3.23 Scardovia wiggsiae F0424S 3.3 3.36 3.12 Actinomyces israelii A87A37 4.65 4.66 4.66 Actinomyces naeslundii I H101A18 3.8 3.82 3.87 Actinomyces naeslundii II H403B5 4.69 4.56 4.68 Peptone Yeast Broth Initial pH of 5.5 Glucose Sucrose Fructose Bacteria Strain pH 5.5 pH 5.5 pH 5.5 Streptococcus mutans ATCC 25175 T 3.95 3.87 3.89 Streptococcus mutans SJ 4.11 4.05 4.06 Streptococcus sobrinus ATCC 27352 T 3.99 4.01 4.03 Streptococcus sobrinus ATCC 33478 3.78 3.71 3.68 Scardovia wiggsiae DSM 22547 / C155A T 3.47 3.65 3.52 Scardovia wiggsiae F0424 S 4.02 3.85 3.75 Actinomyces israelii A87A37 3.85 4.09 4.02 Actinomyces naeslundii I H101A18 4.21 4.21 4.12 Actinomyces naeslundii II H403B5 4.57 4.56 4.59 Open in a separate window T=Type strain S=sequenced strain All other strains are clinical isolates Broth media was prepared using Peptone Yeast base and pH adjusted to pH 7 or pH 5.5 and either unsupplemented or with final concentrations of glucose (1%), sucrose (1%) or sucrose (1%).

Techniques:

Journal: Journal of oral biosciences

Article Title: Scardovia wiggsiae and its potential role as a caries pathogen

doi: 10.1016/j.job.2017.05.002

Figure Lengend Snippet: Based on a protocol for S. mutans [48], 64 25-day-old female Sprague Dawley rats were randomly separated into four groups of 16 rats each: an uninoculated control group and a group each for inoculation by S. wiggsiae alone, S. mutans alone or S. mutans with S. wiggsiae. Rats were housed at two rats per cage, and fed Diet 2000. After 7 weeks infection the experiment was terminated and jaws defleshed by dermestid beetles [48]. Molar teeth (6 mandibular, 6 maxillary) were scored for presence and extent of carious lesions at occlusal, mesial, distal, buccal and lingual surfaces. Lesion extent was measured as: 0=caries free; 1=enamel demineralization or white spots; 2=enamel cavity; and 3=dentinal cavity. The distributions of carious lesions at surface, tooth, and rat level were summarized. Generalized estimating equation models were fit to estimate group differences in caries presence (logit link) or levels (identity link), counting every surface individually. These models accounted for the clustering of outcomes within a rat. Analyses focused on differences attributable to S. wiggsiae.

Article Snippet: S. wiggsiae could thus not mitigate the effects of local acid production in plaque biofilms. table ft1 table-wrap mode="anchored" t5 caption a7 Peptone Yeast Broth Initial pH of 7.0 Glucose Sucrose Fructose Bacteria Strain pH 7.0 pH 7.0 pH 7.0 Streptococcus mutans ATCC 25175 T 3.83 3.76 3.79 Streptococcus mutans SJ 3.86 3.82 3.8 Streptococcus sobrinus ATCC 27352 T 3.72 3.73 3.74 Streptococcus sobrinus ATCC 33478 2.98 2.97 2.91 Scardovia wiggsiae DSM 22547 / C155A T 3.51 3.26 3.23 Scardovia wiggsiae F0424S 3.3 3.36 3.12 Actinomyces israelii A87A37 4.65 4.66 4.66 Actinomyces naeslundii I H101A18 3.8 3.82 3.87 Actinomyces naeslundii II H403B5 4.69 4.56 4.68 Peptone Yeast Broth Initial pH of 5.5 Glucose Sucrose Fructose Bacteria Strain pH 5.5 pH 5.5 pH 5.5 Streptococcus mutans ATCC 25175 T 3.95 3.87 3.89 Streptococcus mutans SJ 4.11 4.05 4.06 Streptococcus sobrinus ATCC 27352 T 3.99 4.01 4.03 Streptococcus sobrinus ATCC 33478 3.78 3.71 3.68 Scardovia wiggsiae DSM 22547 / C155A T 3.47 3.65 3.52 Scardovia wiggsiae F0424 S 4.02 3.85 3.75 Actinomyces israelii A87A37 3.85 4.09 4.02 Actinomyces naeslundii I H101A18 4.21 4.21 4.12 Actinomyces naeslundii II H403B5 4.57 4.56 4.59 Open in a separate window T=Type strain S=sequenced strain All other strains are clinical isolates Broth media was prepared using Peptone Yeast base and pH adjusted to pH 7 or pH 5.5 and either unsupplemented or with final concentrations of glucose (1%), sucrose (1%) or sucrose (1%).

Techniques: Infection

Journal: Journal of oral biosciences

Article Title: Scardovia wiggsiae and its potential role as a caries pathogen

doi: 10.1016/j.job.2017.05.002

Figure Lengend Snippet: Counts of S. wiggsiae and S. mutans from oral swabs of experimental animals

Article Snippet: S. wiggsiae could thus not mitigate the effects of local acid production in plaque biofilms. table ft1 table-wrap mode="anchored" t5 caption a7 Peptone Yeast Broth Initial pH of 7.0 Glucose Sucrose Fructose Bacteria Strain pH 7.0 pH 7.0 pH 7.0 Streptococcus mutans ATCC 25175 T 3.83 3.76 3.79 Streptococcus mutans SJ 3.86 3.82 3.8 Streptococcus sobrinus ATCC 27352 T 3.72 3.73 3.74 Streptococcus sobrinus ATCC 33478 2.98 2.97 2.91 Scardovia wiggsiae DSM 22547 / C155A T 3.51 3.26 3.23 Scardovia wiggsiae F0424S 3.3 3.36 3.12 Actinomyces israelii A87A37 4.65 4.66 4.66 Actinomyces naeslundii I H101A18 3.8 3.82 3.87 Actinomyces naeslundii II H403B5 4.69 4.56 4.68 Peptone Yeast Broth Initial pH of 5.5 Glucose Sucrose Fructose Bacteria Strain pH 5.5 pH 5.5 pH 5.5 Streptococcus mutans ATCC 25175 T 3.95 3.87 3.89 Streptococcus mutans SJ 4.11 4.05 4.06 Streptococcus sobrinus ATCC 27352 T 3.99 4.01 4.03 Streptococcus sobrinus ATCC 33478 3.78 3.71 3.68 Scardovia wiggsiae DSM 22547 / C155A T 3.47 3.65 3.52 Scardovia wiggsiae F0424 S 4.02 3.85 3.75 Actinomyces israelii A87A37 3.85 4.09 4.02 Actinomyces naeslundii I H101A18 4.21 4.21 4.12 Actinomyces naeslundii II H403B5 4.57 4.56 4.59 Open in a separate window T=Type strain S=sequenced strain All other strains are clinical isolates Broth media was prepared using Peptone Yeast base and pH adjusted to pH 7 or pH 5.5 and either unsupplemented or with final concentrations of glucose (1%), sucrose (1%) or sucrose (1%).

Techniques: Cell Counting

Journal: Journal of oral biosciences

Article Title: Scardovia wiggsiae and its potential role as a caries pathogen

doi: 10.1016/j.job.2017.05.002

Figure Lengend Snippet: S. wiggsiae is caries-associated, acidogenic and acid tolerant, but while cariogenicity in animal model was not demonstrated, overall data suggest that S. wiggsiae is a candidate caries pathogen.

Article Snippet: S. wiggsiae could thus not mitigate the effects of local acid production in plaque biofilms. table ft1 table-wrap mode="anchored" t5 caption a7 Peptone Yeast Broth Initial pH of 7.0 Glucose Sucrose Fructose Bacteria Strain pH 7.0 pH 7.0 pH 7.0 Streptococcus mutans ATCC 25175 T 3.83 3.76 3.79 Streptococcus mutans SJ 3.86 3.82 3.8 Streptococcus sobrinus ATCC 27352 T 3.72 3.73 3.74 Streptococcus sobrinus ATCC 33478 2.98 2.97 2.91 Scardovia wiggsiae DSM 22547 / C155A T 3.51 3.26 3.23 Scardovia wiggsiae F0424S 3.3 3.36 3.12 Actinomyces israelii A87A37 4.65 4.66 4.66 Actinomyces naeslundii I H101A18 3.8 3.82 3.87 Actinomyces naeslundii II H403B5 4.69 4.56 4.68 Peptone Yeast Broth Initial pH of 5.5 Glucose Sucrose Fructose Bacteria Strain pH 5.5 pH 5.5 pH 5.5 Streptococcus mutans ATCC 25175 T 3.95 3.87 3.89 Streptococcus mutans SJ 4.11 4.05 4.06 Streptococcus sobrinus ATCC 27352 T 3.99 4.01 4.03 Streptococcus sobrinus ATCC 33478 3.78 3.71 3.68 Scardovia wiggsiae DSM 22547 / C155A T 3.47 3.65 3.52 Scardovia wiggsiae F0424 S 4.02 3.85 3.75 Actinomyces israelii A87A37 3.85 4.09 4.02 Actinomyces naeslundii I H101A18 4.21 4.21 4.12 Actinomyces naeslundii II H403B5 4.57 4.56 4.59 Open in a separate window T=Type strain S=sequenced strain All other strains are clinical isolates Broth media was prepared using Peptone Yeast base and pH adjusted to pH 7 or pH 5.5 and either unsupplemented or with final concentrations of glucose (1%), sucrose (1%) or sucrose (1%).

Techniques: Animal Model

Journal: Scientific Reports

Article Title: Specific detection of interferon regulatory factor 5 (IRF5): A case of antibody inequality

doi: 10.1038/srep31002

Figure Lengend Snippet: ( A ) qRT-PCR analysis of IRF5 transcript expression in immortalized lymphoid cell lines. Data is presented as relative expression after normalization to β-actin using the ∆∆Ct method. ( B ) Immunoblot analysis of endogenous IRF5 protein expression in immortalized cell lines. IRF5 expression was detected with the previously validated Cell Signaling antibody #3257 that is no longer available. β-actin levels and apparent molecular weight standards are shown as loading controls and for size comparison, respectively. ( C ) Experimental details for the comparative analysis of α-IRF5 antibody specificity by immunoblot analysis. ( D ) Same as in ( B )except lysates (T, THP-1; R, Ramos B cells; J, Jurkat T cells) were run on multiple independent blots for the analysis of antibody specificity; seven different commercially available α-IRF5 antibodies were evaluated. ( E ) Same as in ( D ) except antibodies were evaluated by IRF5 knockdown analysis. Ramos B cells were nucleofected with scrambled (Scr) or IRF5 (KD) siRNAs and lysates from same nucleofection run on multiple independent blots for comparative analysis. Arrows indicate detection of an appropriately sized band(s) corresponding to IRF5. Data (except in ( C )) are representative of three independent experiments.

Article Snippet: Membranes were blocked in TBS/0.05% Tween 20 containing 5% BSA for 1 h and incubated overnight at 4 °C with α-IRF5 antibodies (Abcam: #33478, #2932, #124792, #181553; Cell Signaling: #3257, #13496, #4950; Sigma-Aldrich: #SAB1403991; Novus Biologicals: #NB100-1092; Proteintech Group Inc.: #10547-1-AP; R&D Systems: #IC8447P; Santa Cruz: #sc-98651) followed by horse radish peroxidase conjugated secondary antibodies (Cell Signaling: α-rabbit #7074S, α-mouse #7076S; Santa Cruz: donkey α-goat #sc-2020).

Techniques: Quantitative RT-PCR, Expressing, Western Blot, Molecular Weight, Comparison, Knockdown

Journal: Scientific Reports

Article Title: Specific detection of interferon regulatory factor 5 (IRF5): A case of antibody inequality

doi: 10.1038/srep31002

Figure Lengend Snippet: ( A ) Experimental details for the comparative analysis of α-IRF5 antibody specificity by immunoprecipitation. ( B–E ) Lysates from Ramos B cells were immunoprecipitated with the indicated antibodies and IRF5 detected by immunoblot. In ( C–E ), the indicated antibodies were titrated for immunoprecipitation. ( F ) Same as ( B ) except lysates from Jurkat T cells were also immunoprecipitated to test antibody specificity. Arrows indicate detection of an appropriately sized band(s) corresponding to IRF5. Data are representative of three independent experiments.

Article Snippet: Membranes were blocked in TBS/0.05% Tween 20 containing 5% BSA for 1 h and incubated overnight at 4 °C with α-IRF5 antibodies (Abcam: #33478, #2932, #124792, #181553; Cell Signaling: #3257, #13496, #4950; Sigma-Aldrich: #SAB1403991; Novus Biologicals: #NB100-1092; Proteintech Group Inc.: #10547-1-AP; R&D Systems: #IC8447P; Santa Cruz: #sc-98651) followed by horse radish peroxidase conjugated secondary antibodies (Cell Signaling: α-rabbit #7074S, α-mouse #7076S; Santa Cruz: donkey α-goat #sc-2020).

Techniques: Immunoprecipitation, Western Blot

Journal: Scientific Reports

Article Title: Specific detection of interferon regulatory factor 5 (IRF5): A case of antibody inequality

doi: 10.1038/srep31002

Figure Lengend Snippet: ( A ) Experimental details for the comparative analysis of α-IRF5 antibody specificity by flow cytometry. ( B ) Representative histogram plots from flow cytometry analysis of intracellular IRF5 in immortalized cell lines using different α-IRF5 antibodies. Data are representative of three independent experiments. Ctrl-iso, matched isotype antibodies used as non-specific controls.

Article Snippet: Membranes were blocked in TBS/0.05% Tween 20 containing 5% BSA for 1 h and incubated overnight at 4 °C with α-IRF5 antibodies (Abcam: #33478, #2932, #124792, #181553; Cell Signaling: #3257, #13496, #4950; Sigma-Aldrich: #SAB1403991; Novus Biologicals: #NB100-1092; Proteintech Group Inc.: #10547-1-AP; R&D Systems: #IC8447P; Santa Cruz: #sc-98651) followed by horse radish peroxidase conjugated secondary antibodies (Cell Signaling: α-rabbit #7074S, α-mouse #7076S; Santa Cruz: donkey α-goat #sc-2020).

Techniques: Flow Cytometry

Journal: Scientific Reports

Article Title: Specific detection of interferon regulatory factor 5 (IRF5): A case of antibody inequality

doi: 10.1038/srep31002

Figure Lengend Snippet: ( A ) Experimental details for the comparative analysis of α-IRF5 antibody specificity by IHC and IF microscopy. ( B ) Representative core images from IHC analysis of human spleen tissue. IRF5-positive staining is brown, hematoxylin (blue) stains cell nuclei; 2 nd alone, staining with secondary antibody only; RP, red pulp; WP, white pulp. ( C ) Same as ( B ) except tissue cores were double-stained with α-IRF5 antibodies (brown) and α-CD3 antibodies (red). ( D ) Representative images from IF analysis of α-IRF5 (red) and α-CD3 (green) co-staining in human spleen tissue. DAPI (blue) stains cell nuclei. Images were taken at 60X magnification.

Article Snippet: Membranes were blocked in TBS/0.05% Tween 20 containing 5% BSA for 1 h and incubated overnight at 4 °C with α-IRF5 antibodies (Abcam: #33478, #2932, #124792, #181553; Cell Signaling: #3257, #13496, #4950; Sigma-Aldrich: #SAB1403991; Novus Biologicals: #NB100-1092; Proteintech Group Inc.: #10547-1-AP; R&D Systems: #IC8447P; Santa Cruz: #sc-98651) followed by horse radish peroxidase conjugated secondary antibodies (Cell Signaling: α-rabbit #7074S, α-mouse #7076S; Santa Cruz: donkey α-goat #sc-2020).

Techniques: Microscopy, Staining

Journal: Scientific Reports

Article Title: Specific detection of interferon regulatory factor 5 (IRF5): A case of antibody inequality

doi: 10.1038/srep31002

Figure Lengend Snippet: ( A ) Immunoblot analysis of endogenous IRF5 protein expression in primary splenocytes from Irf5 +/+ (WT) and Irf5 −/− (KO) mice. 25 μg protein was separated by SDS-PAGE, as described in the Methods section. β-actin levels and apparent molecular weight standards are shown as loading controls and for size comparison, respectively. Arrows indicate detection of an appropriately sized band(s) corresponding to murine IRF5. ( B ) Representative histogram plots from flow cytometry analysis of intracellular IRF5 expression in splenocytes from Irf5 +/+ (WT) and Irf5 −/− (KO) mice. Ctrl, matched isotype antibodies used as non-specific controls. Data are representative of three independent experiments. ( C ) Representative images from IF (left panels) and IHC (right panels) analysis of IRF5 expression in spleen from Irf5 +/+ (WT) and Irf5 −/− (KO) mice. For IF, IRF5-positive staining is red; DAPI is blue. For IHC, IRF5-positive staining is brown; hematoxylin is blue; 2 nd alone, staining with secondary antibody only.

Article Snippet: Membranes were blocked in TBS/0.05% Tween 20 containing 5% BSA for 1 h and incubated overnight at 4 °C with α-IRF5 antibodies (Abcam: #33478, #2932, #124792, #181553; Cell Signaling: #3257, #13496, #4950; Sigma-Aldrich: #SAB1403991; Novus Biologicals: #NB100-1092; Proteintech Group Inc.: #10547-1-AP; R&D Systems: #IC8447P; Santa Cruz: #sc-98651) followed by horse radish peroxidase conjugated secondary antibodies (Cell Signaling: α-rabbit #7074S, α-mouse #7076S; Santa Cruz: donkey α-goat #sc-2020).

Techniques: Western Blot, Expressing, SDS Page, Molecular Weight, Comparison, Flow Cytometry, Staining

Journal: Scientific Reports

Article Title: Specific detection of interferon regulatory factor 5 (IRF5): A case of antibody inequality

doi: 10.1038/srep31002

Figure Lengend Snippet: IRF5 antibody details.

Article Snippet: Membranes were blocked in TBS/0.05% Tween 20 containing 5% BSA for 1 h and incubated overnight at 4 °C with α-IRF5 antibodies (Abcam: #33478, #2932, #124792, #181553; Cell Signaling: #3257, #13496, #4950; Sigma-Aldrich: #SAB1403991; Novus Biologicals: #NB100-1092; Proteintech Group Inc.: #10547-1-AP; R&D Systems: #IC8447P; Santa Cruz: #sc-98651) followed by horse radish peroxidase conjugated secondary antibodies (Cell Signaling: α-rabbit #7074S, α-mouse #7076S; Santa Cruz: donkey α-goat #sc-2020).

Techniques: Immunohistochemistry-IF